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21.
Shivachandra SB Yogisharadhya R Ahuja A Bhanuprakash V 《Research in veterinary science》2012,93(3):1128-1131
Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (~31kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant. 相似文献
22.
Kumar Antul Sharma Achla Sharma Rajni Choudhary Anuj Srivastava Puja Kaur Harmanjot Padhy Asish Kumar 《Genetic Resources and Crop Evolution》2022,69(1):411-430
Genetic Resources and Crop Evolution - Gradual deterioration in water resources and unpredictable distribution pattern of annual precipitation amount are major threats having drastic effects on... 相似文献